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Advances in the study of directed evolution for cellulases
Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN
《环境科学与工程前沿(英文)》 2011年 第5卷 第4期 页码 519-525 doi: 10.1007/s11783-011-0326-2
关键词: biohydrogen bioethanol cellulase cellulose directed evolution
Development of a dual temperature control system for isoprene biosynthesis in
《化学科学与工程前沿(英文)》 2022年 第16卷 第7期 页码 1079-1089 doi: 10.1007/s11705-021-2088-0
关键词: transcriptional activator directed evolution dynamic control heat-shock isoprene
Unnormalized and normalized forms of gefura measures in directed and undirected networks
Raf GUNS,Ronald ROUSSEAU
《信息与电子工程前沿(英文)》 2015年 第16卷 第4期 页码 311-320 doi: 10.1631/FITEE.1400425
关键词: Networks subdivided in groups Partitions Gefura measures Q-measures Brokerage role Directed and undirected networks Brandes’ algorithm
Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection
Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN
《信息与电子工程前沿(英文)》 2015年 第16卷 第10期 页码 838-847 doi: 10.1631/FITEE.1500069
《化学科学与工程前沿(英文)》 2022年 第16卷 第5期 页码 699-708 doi: 10.1007/s11705-021-2093-3
关键词: layer-by-layer self-assembly single bilayer nanofiltration membrane desalination
《医学前沿(英文)》 页码 878-888 doi: 10.1007/s11684-023-0993-y
关键词: uveal melanoma liver-directed therapy immune checkpoint blockade SIRT anti-PD-1 anti-CTLA-4
RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping
《医学前沿(英文)》 2007年 第1卷 第2期 页码 157-160 doi: 10.1007/s11684-007-0029-z
Site-directed mutagenesis of long QT syndrome KCNQ1 gene
LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing
《医学前沿(英文)》 2008年 第2卷 第1期 页码 100-104 doi: 10.1007/s11684-008-0018-x
关键词: restriction digestion syndrome sequence site-directed mutagenesis
oocyst directed assembly of gold nanoparticles and graphene oxide
Sona Jain, Zhicheng Huang, Brent R. Dixon, Syed Sattar, Juewen Liu
《化学科学与工程前沿(英文)》 2019年 第13卷 第3期 页码 608-615 doi: 10.1007/s11705-019-1813-4
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
《医学前沿(英文)》 2015年 第9卷 第1期 页码 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed gene transfer mediated by adeno-associated viral vectors. Recent clinical trials involving the use of a self-complementary adeno-associated virus serotype 8-human codon-optimized factor IX (AAV8-hFIXco) vector demonstrated encouraging efficacy with hFIX expression stabilized at 1% to 6% of normal level in patients, but safety concerns related to high vector doses are still present. Thus, further improvement of AAV vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B gene therapy. In this study, to obtain a higher expression level of hFIX that potentiates the coagulant capacity of recipients, human FIX expression vector was optimized by upgrading the codon adaption index and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutation, R338L (FIX Padua). The efficiency of the published and the presently constructed cassettes was compared through in vivo screening. In addition, the regulatory elements that control the FIX gene expression in these cassettes were screened for liver-specific effectiveness. Among all the constructed cassettes, scAAV-Pre-hFIXco-SIH-R338L, which was the construct under the control of the prothrombin enhancer and prealbumin promoter, resulted in the highest level of coagulant activity, and the expression levels of two constructed cassettes (scAAV-Chi-hFIXco-SIH-R338L and scAAV-Pre-hFIXco-SIH-R338L) were also higher than that of the published cassette (scAAV-LP1-hFIXco-SJ). In summary, our strategies led to a substantial increase in hFIX expression at the protein level or a remarkably elevated coagulant activity. Thus, these reconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene therapy of hemophilia B.
关键词: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Tuned reactive power dispatch through modified differential evolution technique
S. BISWAS (RAHA), N. CHAKRABORTY
《能源前沿(英文)》 2012年 第6卷 第2期 页码 138-147 doi: 10.1007/s11708-012-0188-8
关键词: reactive power dispatch (RPD) modified differential evolution (MDE) differential evolution algorithm with localizations around the best vector (DELB)
Strategic Thinking on a Revolution/an Evolution in Production and Consumption of Energy in China
Ke-chang Xie,Ke Liu
《工程管理前沿(英文)》 2014年 第1卷 第1期 页码 30-36 doi: 10.15302/J-FEM-2014006
Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang
《医学前沿(英文)》 2019年 第13卷 第3期 页码 354-364 doi: 10.1007/s11684-018-0665-5
关键词: donor leukocyte infusion hematopoietic stem cell transplantation interferon-
Shenghua Ye, Gaoren Li
《化学科学与工程前沿(英文)》 2018年 第12卷 第3期 页码 473-480 doi: 10.1007/s11705-018-1724-9
The polypyrrole(PPy)@NiCo hybrid nanotube arrays have been successfully fabricated as a high performance electrocatalyst for hydrogen evolution reaction (HER) in alkaline solution. The strong electronic interactions between PPy and NiCo alloy are confirmed by X-ray photoelectron spectroscopy and Raman spectra. Because these interations can remarkably reduce the apparent activation energy (Ea) for HER and enhance the turnover frequency of catalysts, the electrocatalytic performance of PPy@NiCo hybrid nanotube arrays are significantly improved. The electrochemical tests show that the PPy@NiCo hybrid catalysts exhibit a low overpotential of ~186 mV at 10.0 mA·cm−2 and a small tafel slope of 88.6 mV·deg−1 for HER in the alkaline solution. The PPy@NiCo hybrid nanotubes also exhibit high catalytic activity and high stability for HER.
关键词: NiCo alloy polypyrrole hybrid nanotube electrocatalyst hydrogen evolution reaction
《化学科学与工程前沿(英文)》 2023年 第17卷 第1期 页码 102-115 doi: 10.1007/s11705-022-2179-6
关键词: oxygen evolution reaction electrocatalysts ternary layered double hydroxides long-term stability
标题 作者 时间 类型 操作
Advances in the study of directed evolution for cellulases
Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN
期刊论文
Unnormalized and normalized forms of gefura measures in directed and undirected networks
Raf GUNS,Ronald ROUSSEAU
期刊论文
Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection
Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN
期刊论文
Ionic strength directed self-assembled polyelectrolyte single-bilayer membrane for low-pressure nanofiltration
期刊论文
Liver-directed treatment is associated with improved survival and increased response to immune checkpoint
期刊论文
Clinical outcomes of ATP-tumor chemosensitivity assay directed chemotherapy in hepatocellular carcinoma
RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping
期刊论文
Site-directed mutagenesis of long QT syndrome KCNQ1 gene
LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing
期刊论文
oocyst directed assembly of gold nanoparticles and graphene oxide
Sona Jain, Zhicheng Huang, Brent R. Dixon, Syed Sattar, Juewen Liu
期刊论文
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
期刊论文
Tuned reactive power dispatch through modified differential evolution technique
S. BISWAS (RAHA), N. CHAKRABORTY
期刊论文
Strategic Thinking on a Revolution/an Evolution in Production and Consumption of Energy in China
Ke-chang Xie,Ke Liu
期刊论文
Minimal residual disease-directed immunotherapy for high-risk myelodysplastic syndrome after allogeneic
Xiaodong Mo, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang
期刊论文
Polypyrrole@NiCo hybrid nanotube arrays as high performance electrocatalyst for hydrogen evolution reaction
Shenghua Ye, Gaoren Li
期刊论文